Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINQKEGDFLRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTPAENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQELKAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTFRTKIRKWKKIVL 2QE9 Chain:A ((14-159)) ---------NLYFQGMMKFFEYNWQVRDQWFTWCHQLTTEELLKNRLGGVENILYTLFHIIDVEYSWIRAIQ----GKEDI---AV-------Q-------FADYQTLNKVKSLSNTFRTEIIDVLQTH------ELVSVPWETGV----LYTRDEILHH-IIAHEIHHIGQLSVWARELK

General information: TITO was launched using: RESULT: Template: 2QE9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 459 -46122 -100.48 -329.44 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -100.48 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2QE9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QE9-query.scw PDB file : Tito_Scwrl_2QE9.pdb: