Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKQTIIVMSDSHGDSLIVEEVRDRYV-GKVDAVFHNGDSELRPDSP-----LWE---GIRVVKGNMDFYAGY----------P-----------------ERLVTEL-----------GSTKIIQTHGHLFDINFN-------FQKLDYWAQEEEAAICLYGHLHVPSAWME-GKILFLNPGSISQPRG------TIRECLYARVEIDD-SYFKVDFLTRDHEVYPGLSKEFSR 3QFM Chain:B ((11-230)) --MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTGRRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEEIEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVYGHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRRVD-------------

General information: TITO was launched using: RESULT: Template: 3QFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 654 -1239 -1.89 -7.84 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -1.89 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_3QFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QFM-query.scw PDB file : Tito_Scwrl_3QFM.pdb: