Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVMQLLPEL----NSGGVERGTLEIARALVAQGHQSLVVSNGGRLVSQLE-----------------------AEGSTHLTLPIHKK-----------------SL-SS-LWQ----IRPLRQLIEEHQPDIVHVRSRVPAWLTHFALRKIPANKHPHLISTVHGFYSVN-----------------------------RYSAIMTQAEKVIAVSDSVVKYITDH-YKNC--------PPQDIVRIYRGIDPAAFPHNYQPS-------------AQWFNQVFNDFPE-LENKFLLCLPGRITRLKGHESLIELMQKLGEQYPQLHAVVVGGADVKKQTYLSELQNTIQSKGLADKI-TFVGHRSDIREWLA-FSDIVLSLSNQAETFGRTALEALSVGTPVIGWNRGGVAEILSHV---------YPQGLVEAENEKALVERVKHHIE---QPQTVA------PVTMFSLKDMCDQTLELYQSVLK 1RZU Chain:B ((1-476)) MNVLSVSSEIYPLIKTGGLADVVGALPIALEAHGVRTRTLIPGYPAVKAAVTDPVKCFEFTDLLGEKADLLEVQHERLDLLILDAPAYYERSGGPYLGQTGKDYPDNWKRFAALSLAAARIGAGVLPGWRPDMVHAHDWQAAMTPVYMR-YA-ETPEIPSLLTIHNIAFQGQFGANIFSKLALPAHAFGMEGIEYYNDVSFLKGGLQTATALSTVSPSYAEEILTAEFG-MGLEGVIGSRAHVLHGIVNGIDADVWNPATDHLIHDNYSAANLKNRALNKKAVAEHFRIDDDGSPLFCVISRLTWQKGIDLMAEAVDEIVS--LGGRLVVLGAGDV---ALEGALLAAASR--HHGRVGVAIGYNEPLSHLMQAGCDAIIIPSR-FEPCGLTQLYALRYGCIPVVARTGGLADTVIDANHAALASKAATGVQFSPVTLDGLKQAIRRTVRYYHDPKLWTQMQKLGMKSDVSWEKSAGLYAALYSQLI-

General information: TITO was launched using: RESULT: Template: 1RZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1832 -32870 -17.94 -92.85 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -17.94 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_1RZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RZU-query.scw PDB file : Tito_Scwrl_1RZU.pdb: