Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIALFYMGGTFGCIGEPL---APMPYDQFLPQLEKVIP---PHLTVDCFAAPNIVDSSACTAPDWLRLIQRIQQLQLE--GYQHFVVIHGTDTLSYAAATLARFLGQSCHIVITGSQYPLLNIQGDNTREFTDAIENLYLALEQVIAL---LVGAYLAFHHQVFHAQTALKTHTTELDAFSGLSSNVEFTPQQN-EL----IVQD----AQIE----KAASFQLLNWMMQPIATQHLVQQLRHLLPAPPHFLVLQGFGTGNIAVNDEFLATLDELYTRGCVPILATQVTFGGI-DQRYAISTWAKTAKIVINDAHSHADLYAKALQIYLKYPTPEQWLNHWNENLH 2WLT Chain:A ((5-331)) LPTIALLATGGTIAGSG--ASLGSYKSGELGVKELLKAIPSLNKIARIQGE-QVSNIGSQDMNEEIWFKLAQRAQE-LLDDSRIQGVVITHGTDTLEESAYFLNLVLHSTKPVVLVGAMRNASSLSA-------DGALNLYEAVSVAVNEKSANKGVLVVMDDTIFSVREVVKTHTTHVSTFKALNSGAIGSVYYGKTRYYMQPLRKHTTESEFSLSQLKTPLPKVDIIYTHAGMTPDL---FQASLNSHAKGVVIAGVGNGNVSAG--FLKAMQEASQMGVVIVRSSRVGSGGVTSGEID----DKAYGFITSDNLNPQKARVLLQLALTKTNDKAKIQEMFEE---

General information: TITO was launched using: RESULT: Template: 2WLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1756 -38322 -21.82 -127.74 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -21.82 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.363

(partial model without unconserved sides chains): PDB file : Tito_2WLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WLT-query.scw PDB file : Tito_Scwrl_2WLT.pdb: