Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDRLQQFYIFTEVAKRQSFSEVANQLDLPRSTVTSAIQQLETHYGVRLFHRTTRKVSLTQDGQRILPECQNLLFDYEQLEQLIQTQKQHYRGTLKISMPSRIVHQVIIPELPDFYHRYPDIHLQLNSSD--DL-TDLIEKGIDCVVRVGELDNSSLIARFIGHLVMVNCASTQYLQDYGIPEQLNDLEQHKLINYAGAVG-E--KQGEFIYQGG-TVMMDSALSVNNTEAYIAAACAGLGIIQLPYYDVQDKIEQG-ILVEVLSAYIAPSLPLNILYPNRSYIPKRLEVFMNWVGEVLNRKCIVIF 4X6G Chain:A ((6-303)) HMTLTELRYIVTLAQEQHFGRAAERCHVSQPTLSVGVKKLEDELGVLIFERSKSAVRLTPVGEGIVAQAQKVLEQAQGIRELAQAGKNQLAAPLKVGAIYTIGPYLFPHLIPQLHRVAPQMPLYIEENFTHILRDKLRTGELDAIIIALPFQEADVLTKPLFDEPFYVLMPADHPWTAKASIDSELLNDKSLLLLGEGHDFRDQVLEACPTVRKGDENKHTTVESSSLETIRHMVASGLGVSVLPFSAVDSHHYAPGVIEVRPFSAPVPFRTVAIAWRASFPRPRAIEVLADSIRLCS--------

General information: TITO was launched using: RESULT: Template: 4X6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1239 -88689 -71.58 -305.82 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -71.58 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_4X6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4X6G-query.scw PDB file : Tito_Scwrl_4X6G.pdb: