Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFSRKQFALSAIFVAILATGGSFMLLHENADAKAAPTAAQQAATVDVAPVVSKTITDWQEYSGRLEAIDQVDIRPQVSGKLIAVHFKDGSLVKKGDLLFTIDPRPFEAELNRAKAQLASAEAQVTYTASNLSRIQRLIQSNAVSRQELDLAENDARSANANLQAARAAVQSARLNLEYTRITAPVSGRISRAEVTVGNVVSAGNGAQVLTSLVSVSRLYASFDVDE-QTYLKYISNQRNSSQV--------PVYMGLANETGFTREGTINSIDNNLNTTSGTIRVRATFDNPNGVLLPGLYARIRLGGGQPRPAILISPTAVGVDQDKRFVVVVDAKNQTAYREVKLGAQQDGLQIVNSGLQAGDRIVVNGLQRIRPGDPVTPHLVPMPNSQITANATPQPQPTDKTSTPAKG 1T5E Chain:D ((29-259)) -----------------------------------------------------------TELPGRTNAFRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQA-------QRYKLLVADQAVSKQQY-------ADANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVTNGQ-ANAMATVQQLDPIY--VDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGSQYPLEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQL-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1T5E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 878 -36445 -41.51 -164.17 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -41.51 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.579

(partial model without unconserved sides chains): PDB file : Tito_1T5E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T5E-query.scw PDB file : Tito_Scwrl_1T5E.pdb: