Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNLNSPEQSDLSWLTIWSTFLNQAPFTAQTQAPEAACFLQQLIEASLQGDSCIDISPEQIETLGQLVISAEQAKSQVAPCVHDGQGLALYRYWNLEQRLAEQIRRLKQQPIQPVSCEEHLDLLTDPHQRTALQMVT-RQSLSIITGGPGTGKTYTLARIIAVLSQAIPHIRVAMAAPTGKAAQRMQEALQNSFNDPKLLESGLMSDELRNQSTQTIHRLLGMGHS---------QTP-RFNQKQPLPYDVIVVDEASMLDLNLATLLFEAVPE---------SCRIILLGDANQLASVDVGAVLADLQQIEA------------LSENRVHLQTSRRFAEGALIGQVARFIQMQTSQIDHEQVLQQFEIDIVQVSELRSIPLEADMPDVVQLEYLPEESPIALETYYQKLMVGFQGYVQSLKAYLQDDHSLEHIENIVKSFDDYRILTAVRHGPFGLQQLNQ-------------YAERWLQQQ---LGIVTLGGWYVGRPVMMTYNDYQLGISNGDIGLCFEHRTQPQQFEVYFPSL-NKWV----------------------AANRLPKSIQSAFALTIHKSQGSEFTHTAVVLDQAAKNLLSQELIYTAITRAKKVVSLLVDPEALLQSFTVRTTRKSGLVEKVNRLVR 5FTF Chain:A ((6-405)) ----------------------------------------------------------------------------------------------------------------------------LTEEMQKIMNLIQDDENNVFVTGKAGSGKTTFLKYLIEKS-----GKNCIVAAPTGIAAINAGGV--------------------------TLHSLFGIPFGPITPYDRLENKFSEYKVECLLKMELLIIDEISMVRPDILDTIDRKLRWVYESDEPFGGVQVIMFGDLFQLPPVTKKQEREILSDFYDGFFFFNALVFKRTGFHIVELTKIFRQTE-PEFINVLNNIRNYQVTSDELDLLSELK---------------------DRKISSS-------------------------------------------YDNEYIHICTHKA---DVEKINADKLGEQEIRNYDIVIKDKFPESSIPCD--LHLKLRVGARVMSLVNDSLKGYYNGMLGIVTALEDN--VITVRMDNGRTIKFERYTWSNTQYTLKDNECVKEEIGSCTQFP-LTLAWAITIHKSQGLTFDKIIIHVSH----TFCPGQLYVALSRCRTLEGIVSDAFI-TK---------------------

General information: TITO was launched using: RESULT: Template: 5FTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1361 -37857 -27.82 -115.07 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -27.82 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_5FTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FTF-query.scw PDB file : Tito_Scwrl_5FTF.pdb: