Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVKNSPAELSSALVNQRNDVPLIRYSGRFAPSPTGPLHFGSLITAVASYCDARAHQGRWLVRVEDTDIPRIYPGSEDHILASLEAFQFDPDAEIIFQKDRLDIYESVLDQLKKEGLVYACQCTRKMLGS----------NAIYAGTCRDLQLD--------FQHQAIRVKVQD-QPICFDDRLQGLHCSNLEHDLGDFVLKRR-----DGIINYQLAVVADDYLQGITHVVRGADLLDNTERQIWLGQLLGYPKLSYMHLPLAMNDQGQKLSKQNLAHALDLTKA----PELLQQAIQALGQPQVD---L-ARPEVMLKQAVTQWNVDLIPHGQQLCGTYL 2CFO Chain:A ((1-310)) ------------------------TVRVRLAPSPTGNLHIGTARTAVFNWLYARHRGGKFILRIEDTDRERSRPEYTENILEGLQWLGLTWDEGPYFQSDRLDLYRQAIQTLLDKGLAYYCYCTPEELEALRAEQKAKGQAPRYDNRHRHLTPEEQAAFEAAGRTPVIRFKIEDDRQIEWQDLVRGRVSWQGADLGGDMVIARAAPRGEIGYPLYNLVVVVDDIAMGITDVIRGEDHIGNTPKQILLYEALGATPPNFAHTPLILNSTGQKLSKRDGVTSISDFRAMGYLAPALANYMTLLGWSPPEGVGELFTLDLA----AKHFSFERINKAGARF----

General information: TITO was launched using: RESULT: Template: 2CFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1314 -37159 -28.28 -133.66 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -28.28 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_2CFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFO-query.scw PDB file : Tito_Scwrl_2CFO.pdb: