Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIKEKTLDDLLNSTYTFILENGDHITA-SKGENFEVRNAVLTLENPRSRLSISE-TRSKLISCIGEFFWYLNGSDSLEFIEYYISDYRKY-IEYQI----------------------------------------------DENKPALGAYGPRIFGA-------QNQFNKIIEVLKDKPTSRRAVISIYSKDDLLREDSRDIPCTCLLQFFIRNERLHLTATMRSNDAAIGLVHDIFSFTLIQELLLAKLIRYIPNLQLGEYTHIVGSLHIYKKI 3UWL Chain:B ((14-263)) --------------------EEGHFKEDRTGTGTYSLFGYQMRFDLA-KGFPLLTTKRVPFGLIKSELLWFLKGDTNIRYLLERNNHIWDEWAFERYVKSADYQGPDMTDFGHRVLQDPAFAEQYKEEHQKFCDAILNDAEFAEKYGELGNIYGAQWRHWETKDGSFIDQLANVIEMIKTNPDSRRLIVSAWNPEDVP--SMALPPCHTMFQFYVNEGKLSCQLYQRSADVFLGVPFNIASYALLTHLIAH-----ETGLEVGEFVHTLGDAHLYQNH

General information: TITO was launched using: RESULT: Template: 3UWL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 586 -28129 -48.00 -144.99 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -48.00 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_3UWL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UWL-query.scw PDB file : Tito_Scwrl_3UWL.pdb: