Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRCNVMKILIIDNYDSFTFNLYQLLGEILISLKSRKEISDFEIVVKRNNEISLDEIRKLEFDRIIISPGPGSPDDERYFGISKQVILQLGKEIPILGVCLGMQGIAYCFGGKVIRAKLPMHGKISTINH-SDSGVFSGIPQ--------DVEVMRYHSLIVDSSTLPECFIVTARVNSAYSDSEEEIMGIKHQTYPIEGVQFHPESFASEAGKTLLQNFVHSKL 1GPM Chain:A ((4-222)) -NIHKHRILILDFGSQYTQLVARRVRELG-----------VYCELWAWDVT-EAQIRDFNPSGIILSGGPESTTEENSP---RAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEASNEREFGYA-QVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTA--IPSDFITVAST------ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRD--

General information: TITO was launched using: RESULT: Template: 1GPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1056 -60527 -57.32 -321.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -57.32 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_1GPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPM-query.scw PDB file : Tito_Scwrl_1GPM.pdb: