Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDSQQSKPKHVMMMAAGTGGHVFPALAVAKQLQQQGCQVSWLATPIGMENRLLKDQNIPIYQIDIQGVR--GN----G--VIRK----LAAPFK-ILKATFSAMRYMKQLKVDAVAGFGGYVAGPGGLAARLLGIPVLIHEQNAVAG---F--------------TN--------------------AQLSR--------------VAKVVCEAFPNTF--PA-S--EKVVTTGNPVRREITDILSPKWRYDEREQ--AGKPLNILIVGGSLGAK---ALNERLPPALKQLQVPLNIFHQCGQQQV-EATQA--LYADAPANLTVQVLPFIEDMAKAYSEADLIICRAGALTVTEVATAGVAAVFVPLPIAVDDHQTANAKFLADVGAAKICQQSTMTPEVLNQLFTTLMNRQLL-TEMAVKARQHAQPNATQHVVGLIQKM 5GL5 Chain:A ((37-455)) ------NKSYKFGLLTIGSRGDVQPYIALGKGLIKEGHQVVIITHSEF-R-DFVESHGIQFEEIAGNPVELMSLMVENESMNVKMLREASSKFRGWIDALLQTSWEVCNRRKFDILIESPSA--MVGIHITEALQIPYFRAFTMPWTRTRAYPHAFIVPDQKRGGNYNYLTHVLFENVFWKGISGQVNKWRVETLGLGKTNLFLLQQNNVPFLYNVSPTIFPPSIDFSEWVRVTGYWFLDDKSTFKPPAELQEFISEARSKGKKLVYIGFGSIVVSNAKEMTEALVEAVMEA--DVYCILNKGWSERLG----KKTEVDLPRN--ILNIGNV-PHDWLFPQVDAAVHHGGSGTTGASLRAGLPTVIKPFFG----DQFFYAGRVEDIGVGIALKKLN--AQTLADALKVATTNKIMKDRAGLIKKKISKEDGIKTAISAI---

General information: TITO was launched using: RESULT: Template: 5GL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1536 -57473 -37.42 -170.54 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -37.42 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_5GL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GL5-query.scw PDB file : Tito_Scwrl_5GL5.pdb: