Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRIYVRASTKDQDAERALADLKNFALSIKGDFNEYIENESGTKLDRPVLNQLLDDSSNGDTLLVESVDRLSRLSQNDFEVLKGRIKDKGLKLVVADLPTTHMLVNSADNITSSILSLVNNMLIDLLATMARLDNDKRRERIKQGLERSGYKPTGKKADTAKHTRIKELNNKGLTKEEIAKAVGCGVATVYRVIKK 2GM5 Chain:C ((2-125)) -ALFGYARVS-----LDIQVRALKDAGVKAN---RIFTDKAS---SDRKGLDLLRMKVKEGDVILVKKLDHLGRDTAD-MIQLIKEFDAQGVSIRFIDDGIST-------------DSYIGKMVVTILSAVAQAERQRILER-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 482 -39695 -82.35 -342.19 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -82.35 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_2GM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM5-query.scw PDB file : Tito_Scwrl_2GM5.pdb: