Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDPK-----LIAQADKIVFPGVGAMRDC--MQGMREAGIDEVVRKAAFNKPVLAICVGMQALLQSSEENGGVDALGIFDGVVKHFPQMEGLKVPHMGWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDE-NLVAATCEYGVNFCTAI-HKDNLFATQFHPEKSH-TAGLQLLKNFVEWNI 2VXO Chain:B ((30-222)) -GAVVILDAGAQYGKVIDRRVRELFVQSEIFPLETPAFAIKEQGFRAIIISGGPNSVYAEDAPWF-----DPAI--FTIGKPVLGICYGMQMMNKVFG------------GTVHKKS---------DGVFNISVDN-TCSLFRGLQKEEVVLLTHGDSVDKVADGFKVVARSGN--IVAGIANESKKLYGAQFHPEVGLTENGKVILKNFLY---

General information: TITO was launched using: RESULT: Template: 2VXO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 919 -31404 -34.17 -184.73 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -34.17 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_2VXO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VXO-query.scw PDB file : Tito_Scwrl_2VXO.pdb: