Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKDERIIQNQDNKKTIRHEDYIAGIGKGMAILDSFSSNQHRLKISMAAEKTGLTRAAARRHLLTLEYLGYLESD-GHYYYLTPKVLKFSGAYLGAAQLPKVSQPLLNLLTNQTSLIYSVMVLDGYEAITIARSAAHQQTDRVNPYGLHLGNRLPAHATSAGKILLAHLSQEEQLDWLQKYPLQRLTKYTHVENDKFLELLKEIREQDWCYSREEHELGFHALAVPIYGQDFKVVAALNIVSPTMRTTEGYLIQYILPLLQETARDLRQVL-- 1MKM Chain:A ((4-249)) ----------------------MNTLKKAFEILDFIVKNPGDVSVSEIAEKFNMSVSNAYKYMVVLEEKGFVLRKKDKRYVPGYKLIEYGSFVLRRFNIRDIAHDHLVDIMKRTGETVHLILKDGFEGVYIDKV----EGEQSIPMVSRLGMKVDLYSTASGKSILAFVPEKELKEYLKIVELKPKTPNTITNPRVLKRELEKIRKRGYAVDNEENEIGIMCVGVPIFDHNGYPVAGVSISGVARKFTEEKIEE-YSDVLKEKAEEISRKLGY

General information: TITO was launched using: RESULT: Template: 1MKM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1157 -44548 -38.50 -183.33 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -38.50 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1MKM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MKM-query.scw PDB file : Tito_Scwrl_1MKM.pdb: