Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPAIRRTVTFPEISMERLKSILGAYNGHLKQIEQRLDVKITHRGDVFYIDGEIDAVGRAEALLQRLYEETEASQQISADLLHLLIQSSQTERNFELVGEEMDEHDAPMDVYFQTRKGRINPRGANQKRYVQRILQSDISFGVGPAGTGKTYLAVAAAVDMLERNEIQRILLVRPAVEAGEKLGFLPGDLTQKIDPYLRPLYDALYEMLGFEKVAKLIERQVIEVAPLAYMRGRTLNHSFVILDEAQNTTPEQMKMFLTRLGF---------GSRAVITGDITQVDLPRGQQSGLAHALRVLEN-IKEIHITRFHSRDVVRHQLVQKIVEAYEAWDGEQQRLNAEARAERKARQEALIAENDTAADLQHQDA 5FHH Chain:A ((6-193)) ------------------------------------------------------------------------------------------------------------------------PQLSEEQAAVLRAVLKGQSIFFTGSAGTGKSYLLKRILGSLPP----TGTVATASTGVAACHIGG--T-----------TLHAFAGIGSGQAPLAQC-----VALAQRPGVRQGWLNCQRLVIDEISMVEADLFDKLEAVARAVRQQNKPFGGIQLIICGDFLQL--P-------PPRFCFQSKSWKRCVPVTLELTKVWRQA-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FHH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 780 -66468 -85.22 -410.30 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -85.22 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_5FHH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FHH-query.scw PDB file : Tito_Scwrl_5FHH.pdb: