Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIREILLADTHNYYGILDERFIDLAHQFSRLQDARTGQGGAALAVYFRGQKVVDIYTGLK--SQTEAWQPDTLAVCYSTGKGVLATLAHILVSEGFLEYDKPIATYWPEFA----QNGKEQMTLRHVLSHQSGM-FDVRNIIESAREML-----------DWSHMLVVVAATKPRFLAGEGNAYQALTFGWLVGGVLEKATGQSLDQLMQNYLVEPLQLDGAYFGTPANELDRVARLIIQPKPEKPASTQVEKPKKPQTRKSSLSEKVITWTGQDPQDFQDAMIPKGMKNFSFFSDEGLQAVIPAANGTFTANSLAKIYAMLANQGEWDGQQLIRPEVFKELSTIQSYARDRVMPIPMNWRLGYHRIITMGK----RAKNGFGHIGYNGSGAWCDPERDLSFAYTHNLQIGSITGDYRLWGLTQEALRCTDQILKGRKGWF 1CI9 Chain:A ((15-391)) ----------------AASLAARLDAVFDQALRERRLV-GAVAIVARHGEILYRRAQGLADREAGRPMREDTLFRLASVTKPIVALAVLRLVARGELALDAPVTRWLPEFRPRLADGSEPLVTIHHLLTHTSGLGYWLLEGAGSVYDRLGISDGIDLRDFDLDENLRRLASAPLSFAPGSGWQYS--LALDVLGAVVERATGQPLAAAVDALVAQPLGMRDCGFVSA--EPERFAVPYHDGQP--EPVRMRDGIEVPLPEGHGAAVRFAPSRVFEPGAYPSG----------------------GAGMYGSADDVLRALEAIRA-----NPGFLPETLADAARRDQAGVGAETRGPGWGFGYLSAVLDDPAAAGTPQHAGTLQWGGVYGHSWFVDRALGLSVLLLTNTAYEGMSG---------PLTIALRDAVYA-----

General information: TITO was launched using: RESULT: Template: 1CI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2045 1324 0.65 3.73 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 0.65 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_1CI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CI9-query.scw PDB file : Tito_Scwrl_1CI9.pdb: