Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGTPMTVIPTIDIVDALAAEYAAKSPREILELALSQQGEIAISFSGAEDV-VLIDMASRLG----KPFRVFSLDTGRLHPETYQFFETVRKHYNINIEICFPDAEAVQSMVNEKGLFSFFKDGHQECCGIRKVQPLRKKLA--TLDGWITGQRKDQSPGTRTEIPVVQADA---GFS---------------GPGKQLIKYNPLANWSSADVWSYIRMMEIPYNPLHER-GFVSIGCEPCTRPVLPNQHEREGRWWWEEATQKECGLHAGNLKK 1ZUN Chain:A ((32-231)) -----------------------EAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVMHVDTRWKFQEMYRFRDQMVEEMGLDLITHIN---------------------SAKHTDIMKTEGLKQALDKHGFDAAFGGARRDEEK-SRAKERVYSFRDSKHRWDPKNQRPELWNVYNGNVNKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAA--------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 630 7571 12.02 47.32 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 12.02 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_1ZUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZUN-query.scw PDB file : Tito_Scwrl_1ZUN.pdb: