Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIFDLHIQVQPQHIDALGHVNNVMYVQWMQDVAAAHVETLGVGVTKYLELKHAMVAVEHHVQYRKAAFEGEEIVLRTWLDDINALY-SFRQYAFFRPSDQAILFVGNTKWACIEIASGRPKRMSPTFTQAYKPLDSSINPLDFTVSYA 5EO4 Chain:B ((2-140)) IKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYSHGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYD-YDYKRLHLFLTLTK-EDGTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQP---------

General information: TITO was launched using: RESULT: Template: 5EO4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 608 -50062 -82.34 -362.77 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -82.34 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_5EO4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EO4-query.scw PDB file : Tito_Scwrl_5EO4.pdb: