Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMTFIVAVQLKDSIVVAVDNKYLTLKDKEKDHFEEHFSSKLYAWHSGIITGTGEHYVIDKAVRLFINSADADIEKLPLCLNISRQIREMEVGQHEQIQSSKLLYSQYSENGAKLFAIEPTEETGKYQRTEFKENDLIIWLFNPNIQSISGNLKTLYS-NPRPKVSFDRIEDWLDYYISAIAEIYEKQSCIDSWMSGSFDIFFQTEGEYFYKHIQNNPTVHLE 4DX8 Chain:H ((13-202)) -----------EDAYVAVIRPKSLNSREYRAKSYEILLHEVPK-------KVLLETKLQSEITQGILDYVVETTKPINQGIRGKRVVLMKKFPLD----------GEKMGREASLFIVPSVVC--------------------PIFYCLQDIMRVCTHFATLTARMLIALDKWLDERHAQ---------------SHFIPALFRPSPLERIKTNVINPAYAT-

General information: TITO was launched using: RESULT: Template: 4DX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 670 -39707 -59.26 -251.31 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain H : 0.57 3D Compatibility (PKB) : -59.26 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_4DX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DX8-query.scw PDB file : Tito_Scwrl_4DX8.pdb: