Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYFAAPMFAKS-DLLYNQQLVKEIRELSSELSIYLPQENEAIND-KTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTDTRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIK--LNGKVVSSEIELLSEVKRFLDGGTFSD 4P5E Chain:A ((3-145)) PALYFCGSIRGGREDRTLYERIVSRLRR--FG-TVLTEHVAA----GEEAAGGDRLIHEQDLEWLQQADVVVAEV--TQPSLGVGYELGRAVAFNKRILCLFRPQSG--------------------RVLSAMIRGAADGSRFQVWDYEEGEVEALLDRYFEADPL--

General information: TITO was launched using: RESULT: Template: 4P5E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 602 -27780 -46.15 -208.87 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -46.15 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_4P5E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P5E-query.scw PDB file : Tito_Scwrl_4P5E.pdb: