Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -TQKCIITIRKADPALGQDTLYDLVSLAPNSDLTFGKIPASMTEVTF-GVDEKCQA--RLVRGDLSRPYEYKGMQNPLYGLDGPDFVKKLQAWRRKPENSINFSLW----- 1MFG Chain:A ((1-95)) GSMEIRVRVEK-DPELG-------FSISGGVGGRGNPFRPDDDGIFVTRVQPEGPASKLLQPGD--KIIQANGY--SFINIEHGQAVSLLKT----FQNTVELIIVREVSS

General information: TITO was launched using: RESULT: Template: 1MFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 356 20405 57.32 237.26 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 57.32 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_1MFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MFG-query.scw PDB file : Tito_Scwrl_1MFG.pdb: