Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------KCNISILKDGKYFTQ----KQEIAGW-------TVKFHDLDGN-----YVGKCKNAHTYACTYVTCE----DLAP---GYSPGKSKDVVASG-------NVGSLGHDPSSSSPSSP-------- 1P5U Chain:B ((1-149)) ADLTASTTRTATLVEPARITLTYKEGAPITIMDNGNIDTELLVGTLTLGGYKTGTTSTSVNFTDAAGDPMYLTFTSQDGNNHQFTTKVIGKDSRDFDISPKVNGENLVGDDVVLATGSQDFFVRSIGSKGGKLAAGKYTDAVTVTVSNQ

General information: TITO was launched using: RESULT: Template: 1P5U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 135 2089 15.47 24.29 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 15.47 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.162

(partial model without unconserved sides chains): PDB file : Tito_1P5U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P5U-query.scw PDB file : Tito_Scwrl_1P5U.pdb: