Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MVAGLTNGELIAPMTYEEMVTSDFFEAWFQKFLLPTLTTPSVIIMDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIE-KTWAYIKKNLKKVLPSCNTFYEALFSCSCFN----------------------------- 3HOT Chain:A ((5-345)) VPNKEQTRTVLIFCFHLKKTAAESHRMLVEAFGEQVPTVKTCERWFQRFKSGDFDVDDKEHGKPPKRYEDAELQALLDEDDAQTQKQLAEQLEVSQQAVSNRLREMGKIQKVGRWVPHELNERQMERRKNTCEILLSRYKRKSFLHRIVTGDEKWIFFVNPKRKKSYVDPGQPATSTARPNRFGKKTMLCVWWDQSGVIYYELLKPGETVNAARYQQQLINLNRALQRKR---PEYQHRVIFLHDNAPSHTARAVRDTLETLNWEVLPHAAYSPDLAPSDYHLFASMGHALAEQRFDSYESVKKWLDEWFAAKDDEFYWRGIHKLPERWEKCVASDGKYFE

General information: TITO was launched using: RESULT: Template: 3HOT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 279 -11166 -40.02 -102.44 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -40.02 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_3HOT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HOT-query.scw PDB file : Tito_Scwrl_3HOT.pdb: