Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGYPLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNGNFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGD--HVDHVFVNSIGRALEFMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILIIWKSVEKLLNNIKELYLRNGAAYSIRFWIKK---------------------- 5CGZ Chain:A ((1-240)) -----MKSALVVSAHSADFVWRAGGAIALHAEQGYAMHVVCLSFGER-----GESAKLWRKGEMTEAKVKDARREEAMAAAEILGASVEFFDIGDYPMRADKDTLFRLADVYRRVQPE---------FVLSH-----------SLKDPYNYDHPLAMHLAQEARIIAQAEGYKPGEKIVGAPPVYAFEPHQPEQCEWRPD-----TFLDITSVWDKKYAAIQCMAGQEHLWEYYTRVALQRGVQAKRNVGITSARNIVYAEGLQSVFPRVTENLA

General information: TITO was launched using: RESULT: Template: 5CGZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1102 -68367 -62.04 -316.51 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -62.04 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_5CGZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CGZ-query.scw PDB file : Tito_Scwrl_5CGZ.pdb: