Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLVKEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGSGNRNFNNQYCLTAKQYSQRFGFPVLAD-FEM-----RGMLEDIKHVAAIIADLYELEKEN 1E5D Chain:A ((2-402)) QATKIIDGFHLVGAIDWNSRDFHGYTLSPMGTTYNAYLVEDEKTTLFDTVKAEYKGELLCGIASVIDPKKIDYLVIQHLELDHAGALPALIEACQPEKIFTSSLGQKAMESHFHYKDWPVQVVKHGETLSLGKRTVTFYETRMLHWPDSMVSWFADEKVLISNDIFGQNIAASERFSDQIPVHTLERAMREYYANIVNPYAPQTLKAIETLVGAGVAPEFICPDHGVIFRGADQCTFAVQKYVEYAEQKPTNKVVIFYDSMWHSTEKMARVLAESFRDEGCTVKLMWCKACHHSQIMSEISDAGAVIVG-SPTH--------NNGILPYVAGTLQYIKGLRPQNKIGGAFGSFG--WSGESTKVLAEWLTGMGFDMPATPVKVKNVPTHADYEQLKTMAQTIARALKAKLAA

General information: TITO was launched using: RESULT: Template: 1E5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 693 18674 26.95 128.78 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 26.95 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1E5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5D-query.scw PDB file : Tito_Scwrl_1E5D.pdb: