Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVAAQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENF--LTHAKEGY-YNGITFHRVIDGFMVQTGD-PKGDGTGGQSIWHDKDKTKDKGTGFKNEITPYLYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLDGKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS 2CFE Chain:A ((16-162)) ----------------------------------------------------------------------------------GTIKFKLFDDVVPKTAANFRALCTGEKGFGYAGSHFHRVIPDFMLQGGDFTAGNGTGGKSIY---------GAKFADENFQLKHNKPGLLSMANAG-PNTNGSQFFITT-----------------VVTSW-------LDGKHVVFGEVIDGMNVVKAI-EAEGSGSGKPRSRIEIAKCGVC-------

General information: TITO was launched using: RESULT: Template: 2CFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 713 4234 5.94 29.61 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 5.94 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_2CFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFE-query.scw PDB file : Tito_Scwrl_2CFE.pdb: