Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINM---TGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKL--NIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEEL---NHEEVVEVTERVKGDFKGLLKAILAEL 1V2H Chain:E ((12-281)) ---------TAEWLLSHTKHRPQVAIICGSGLGGLTDKLTQAQIFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMMQGRFHMYEGYPLWKVTFPVRVFHLLGVDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPGFSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSFETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLEKANHEEVLAAGKQAAQKLEQFVSILMASI

General information: TITO was launched using: RESULT: Template: 1V2H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1426 -85998 -60.31 -330.76 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.83 3D Compatibility (PKB) : -60.31 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_1V2H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V2H-query.scw PDB file : Tito_Scwrl_1V2H.pdb: