Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVPKTATSTETKTITRIIHYVDKVTNQNVK----EDVVQPVTLSRTKTENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPD---------------------------------------IPTVESQEVTSDSSDKEITVRYDRLSTP------------EKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLA-SAGIMTLLAGLGLGFFKKKEDEK 1FOU Chain:A ((11-285)) SINEIQRQKRNRWFIHYLNYLQSLAYQLFEWENLPPTINPSFLEKSIHQFGYVGFYKDPVISYIACNGALSGQRDVYNQATVFRAASPVYQKEFKLYNYRDMKEEDMGVVIYNNDMAFPTTPTLELFAAELAELKEIISVNQNAQKTPVLIRANDNNQLSLKQVYNQYEGNAPVIFAHEALDSDSIEVFKTDAPYVVDKLNAQKNAVWNEMMTFKLQTDEQIDSSGTVFLKSREEACEKINELYGLNVKVKFRYDIV-

General information: TITO was launched using: RESULT: Template: 1FOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 653 41942 64.23 208.67 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 64.23 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.119

(partial model without unconserved sides chains): PDB file : Tito_1FOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FOU-query.scw PDB file : Tito_Scwrl_1FOU.pdb: