Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKCADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAVELDYKGIRLE---SPDELLSYFEMNGFVDEEATLL---YATSQGYSMIWFNPFYLEEQ- 1MK4 Chain:A ((0-156)) ---HMDIRTITSSDYEMVTSVLNEWWGGRQLK-EKLPRLFFEHFQDTSFITSEHNSMTGFLIGFQSQSDPETAYIHFSGVHPDFRKMQIGKQLYDVFIETVKQRGCTRVKCVTSPVNKVSIAYHTKLGFDIEKGTKTVNGISVFANYDGPGQDRVLFVKNI

General information: TITO was launched using: RESULT: Template: 1MK4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 593 -55359 -93.35 -369.06 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -93.35 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_1MK4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MK4-query.scw PDB file : Tito_Scwrl_1MK4.pdb: