Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIRCDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDI-PKIWGTYRQMIEGHGTDLKTITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV 2WCV Chain:I ((1-138)) MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIRADGLLVSDLLQAIIPLFELDSYAPPLV-MMAAVEGDTLDPEVERRYRNALSLQAP-CPDIIRINRFAFYERAQKAFAIVITGERAKYGNILLKKGV-------

General information: TITO was launched using: RESULT: Template: 2WCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 671 -77342 -115.26 -564.54 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain I : 0.84 3D Compatibility (PKB) : -115.26 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_2WCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WCV-query.scw PDB file : Tito_Scwrl_2WCV.pdb: