Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKRNNSQNLIMLKHDFASHIEDLKCATKPRSEALGRHLWTCT-------IDADFYWLKFHLPNVHAQSEQDFLHELQFYEDITYKKANWLLPFKIIEGRTISQQTQFQGKVLV--LPDTECWFDDL---DQKQNLKNINEKIYLALVKLAELHELGWIHGDIKKEHFRKFK-QELYLIDFEKTRLISSP-----DPITDATPRYMAPELFHGANKTVQSDLYALGIVLYEWLT-QTRLQANSYHEWAVLH-CQKLNVELPSS-FQIFLPLLSGLLQKQQQNRFSNVHEAINCLKALST 3ZZW Chain:A ((4-288)) ----------------KEISLSAVRFMEELGEDRFGK-VYKGHLFGPAPGEQTQAVAIKTLKDKAEGPLREEFRHEAMLRARLQH--PNVVCLLGVVTK--------DQPLSMIFSYCSHGDLHEFLVMR-SALEPPDFVHLVAQIAAGMEYLSSHHVVHKDLATRNVLVYDKLNVKISDLGLFREVYAADYYKLLGNSLLPIRWMAPEAIMYGKFSIDSDIWSYGVVLWEVFSYGLQPYCGYSNQDVVEMIRNRQVLPCPDDCPAWVYALMIECWNEFPSRRPR-FKDIHSRLRAWG-

General information: TITO was launched using: RESULT: Template: 3ZZW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1160 -7349 -6.33 -29.63 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -6.33 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_3ZZW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZZW-query.scw PDB file : Tito_Scwrl_3ZZW.pdb: