Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTLAERLRYAMEVLPPKKIKGVELARAVGVKPPSVSDWLSGKSKTMEGENLLRASKFLNVNPSWLASGTGEIQTSTKDKFKQLDIEEFKKKYNISDSDEALLFSTIIEKPFIPSSKRWVPVKAYSKMGMDGYFTDIGYEGNAGDGYVPTHTAGSRAYAVKGTGDSMFPAIRN--GW--------YVVCDPDADLVPNEFVQVCLKDGRCTIKEFVGINGGVLSLLSVNGGERFFFEMDEVESITAITDIVPPSQHRQEHPYSH 4Y42 Chain:G ((13-156)) --TLTDTIM---MAKIRKNLTFEAINQGTGLSLAFVTAALLGQHPL-PEQAARVVAEKLDLDEDAIRLLQTI------------------------------------------------PLRGSIPGG---VPTD------------P--TIYRFYEMVQIYGSTLKALVHEQFGDGIISAINFKLDIKKVPDPDGGERAVITLDGKYLPTKPF-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Y42.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 366 -27062 -73.94 -201.95 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain G : 0.57 3D Compatibility (PKB) : -73.94 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_4Y42.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y42-query.scw PDB file : Tito_Scwrl_4Y42.pdb: