Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLSVMQSLQSLQPRISVAPMMDWTTKDYRFFARLFNPNVVLYTEMVTTGAILFGDA--KRHLDYNAQEHPIVLQLGGSNPQELATCTKMAEDWGYDEVNLNVGCPSDRVQNNKIGACLMAEPDLVAECIHSMQKAVNIPVTVKHRIGIDDMQSYEEMLHFVDTVAATGCTHFVVHARIAILKGLSPKENREVPPLRYEDVYRLKQERPHLTIEINGGIKTFAETQAHL-Q-HVDGVMIGREAYHNPYLLAELGQ-LWNL----EA-PDRFDIMEQMLPYIEQRLAEGAPLSIITRHILGLFQNLPGARKWRQALSGGNAKTLTDVENAIHNMQAAIIRTEEYLKEHQF 1VHN Chain:A ((5-309)) -------------VKVGLAPMAGYTDSAFRTLAFEWG-ADFAFSEMVSAKGFLMNSQKTEELLPQP-HERNVAVQIFGSEPNELSEAARILSE-KYKWIDLNAGCPVRKVVKEGAGGALLKDLRHFRYIVRELRKSVSGKFSVKTRLGWEK----NEVEEIYRILVEEGVDEVFIHTRTVVQ-------SF-TGRAEWKALSVLEK---RIPTFVSGDIFTPEDAKRALEESGCDGLLVARGAIGRPWIFKQIKDFLRSGKYSEPSREEILRTFERHLELLIKTKGERKAVVEMRKFLAGYTKDLKGARRFREKVMKIE--EVQILKEMFYNFIKEVE-----------

General information: TITO was launched using: RESULT: Template: 1VHN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1602 18590 11.60 63.02 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 11.60 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_1VHN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VHN-query.scw PDB file : Tito_Scwrl_1VHN.pdb: