Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVVAQQLGQWAEQTALKLLKEQNYEWVASNYHSRRG----EVDLIV-----KRGNELIFVEVKARGQGNYGQACEMVTLSKQKKIIKTAMRFLQRYPSYQDFYCRFDVICFDFPQKIAKTVQQDFSKFHYDLQWIENAFTLD 2OKF Chain:A ((8-65)) ----------FHEVVKTALKKDGWQITDDPLTISVGGVNL------KLIAAERQGQKIAVEVKSFLK---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2OKF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 91 -3693 -40.58 -87.92 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -40.58 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.672

(partial model without unconserved sides chains): PDB file : Tito_2OKF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OKF-query.scw PDB file : Tito_Scwrl_2OKF.pdb: