Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTSTTSTVPLEPWIAQQLQQATQGYWHK-DQIPQSEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVAANGCQVAIVERPIDAEIAQLVVADTRLALGQLGAYRREQ-NAQLKVIALTGSSGKTTTKEMLGSILSRLAPT---LINR-----G-NLNNDLGVP--MMLLEL-----RKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIREAAKSHQIMSFGAG--GDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQK---APHLFIDDTYNANPTSMRAAAQVLLQQ-NGIKVMVMGDIGELGDSSWQEHHD-LGRDLAGLPLDHIVAVGQF-----ASAALEGAGLHS---TKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL 4BUB Chain:B ((1-461)) -------------MNISTIVSNLKDLILEVRAPYDLEITGVSNHSSKVKKGDLFICRRGE--DSHEIIPEVMEKGAVAVVVEREIDLDFPYIQVFDSRYFEAKVASLFFEDPWKDVLTFGVTGTNGKTTTTMMIYHMLTSLGERGSVLTTAVKRILGNSYYDDITTPDAITILSAMKENREGGGKFFALEVSSHAL--VQQRVEGVRFDVGIFTNISRDHLDFHGTFENYLKAKLHLFDLLKDDGVAVLNESLADAF--NR---KSRKITFGTSKNADYRLGNIEVSWEGTQFVLETPDGLLKVFTRAIGDFNAYNAAAAIAALHQLGYDPKDLASSLETFTGVEGRFEVVRGAKKIGLNVVVD-FAHSPDALEKLLKNVRKISQGRVIVVFGAGGNSD----RGKRPMMSEVASKL-ADVVILTTDDPRGEDPEQIMEDLIKGIDKRKPYLVLFDRREAIETALTIA----NRGDSVVIAGRGHERYQII--------

General information: TITO was launched using: RESULT: Template: 4BUB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2312 2355 1.02 5.53 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 1.02 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4BUB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BUB-query.scw PDB file : Tito_Scwrl_4BUB.pdb: