Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNHFATELDELKQQGNFRQFT--QNVQHGRFITIQNKTMLNLASNDYLGLAADINLRQEFLDSYPLERSYFSSSSSRLLTGNFDEYEQLENSLSQAFSGRAALLFNSGYHMNIGILPAVADSKTLILADKLVHASMIDGIRLSGAQYVRYRHNDLQHLEQLLQKYHDDDQIERIIVVTESIFSMDGDETDLTALVQLKKRFAKTMLYVDEAHSIGVRGQQGLGCAEQYNVIQEIDFLVGTLGKALAAVGGYIICDQIIKDYLINKMRPLIFSTAQPPIVMAWANFIFKKVLMA--QSQREHLKNISQYLQQAVVQKGYVS-PSTSHIIPVIVGESQATIDKAKQVQESGFYAMPVRPPTVPKNSSRLRISLTSMVEQHELEALVSFL 1BS0 Chain:A ((2-380)) -SWQEKINAALDARRAADALRRRYPVAQGA-GRWLVADDRQYLNFSSNDYLGLSHHPQIIRAWQQ--GAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRALLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLARLLASPC----PGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHN-GWLMVDDAHGTGVIGEQGRGSCWLQKV--KPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQALRASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQLAEKLRQQGCWVTAIRPPTVPAGTARL-LTLTAAHEMQDIDRLLEVL

General information: TITO was launched using: RESULT: Template: 1BS0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2096 -19968 -9.53 -53.53 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -9.53 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_1BS0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BS0-query.scw PDB file : Tito_Scwrl_1BS0.pdb: