Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQVYLVGGAVRDYLLGHSYQEKDYVVVGATPEHMLAQGFQP--VGKDFPVFLH--PETKEEYALARTERKSGQGYHGFQFFTDTTVSLEDDLIRRDLTINAIAMDQDGKIYDPYGGQNDLENKILRHVSE---AFAEDPLRVLRVARFAARYFPYGFQIAPETLQLMQTMADSGELDALTPERVWKETSRALMENHADIYFQTLRDCGALKHLFPEIDALFGVPQRPEYHPEVDCGIHTLMSLQQACKSNYSLDVRFAVLVHDLGKALTPAKELPRHIMHEERGIKPVTQLCERLRVPTQTKQLALSVC---KEHLKCHQIMSLKPGTLWRLLQRLDVLRRPERVEAFVQACECDAKGRLGLE--DRPYP-QAQYMREA---MQIVRSIKVQ-DLP-ENI-KGAEIGEMLIQYRIEALAEFKNQHQSLSHS 1MIW Chain:A ((21-388)) -DAYFVGGAVRDLLLGRPIGDVDIATS-ALPEDVMAIFPKTIDVGSKHGTVVVVHKGKAYEVTTFKTDG-----------SVTFVRSLEEDLKRRDFTMNAIAMDEYGTIIDPFGGREAIRRRIIRTVGEAEKRFREDALRMMRAVRFVSEL---GFALAPDTEQAIVQNA--PLLAHISVERMTMEMEKLLGGPFAARALPLLAETGLNAY-LPGLAGKEK--Q---LR---------LAAAYRWP-WLAAREERWALLCHALGVQE-------------------SRPFLRAWKLPNKVVDEAGAILTALADIPRPEAW---TNE---QLFS-AGL----ER-----ALSVETVRAAFTGAPPGPWHEKLRRRFASLPIKTKGELAVNGKDVIEWVGKPAGPWVKEALDAIWRAVVNGEVENEKER---

General information: TITO was launched using: RESULT: Template: 1MIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1572 -763 -0.49 -2.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -0.49 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_1MIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MIW-query.scw PDB file : Tito_Scwrl_1MIW.pdb: