Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIGYARTTINDVDLTTQVDQLSDFGCQ--EIFHETYEVEQNDSTAVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTR-EMGKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKKETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI 2GM5 Chain:C ((2-125)) -ALFGYARVS-----LDIQVRALKDAGVKANRIFTDKAS----S-DRKGLDLLRMKVKEGDVILVKKLDHLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDSYIGKMVVTILSAVAQAERQRILER---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 482 -6949 -14.42 -61.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -14.42 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_2GM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM5-query.scw PDB file : Tito_Scwrl_2GM5.pdb: