Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPGPRDALKHMLAYGERDGFK-VKNVD-------NY------AGHI-DLG--EGDETLGIFGHMDVVPAG--DGWDTDPYEPVIK--DGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMAYYFEH-E----EEPDFGFSPDAEFP------IINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVPGTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGA--HGASPQ-SGINAGSFLALFLDNYEFLGS---AK---QFIHVAAAY---VHEDFYG--EK----LGV---------AYEDEKMGKLTMNAGLFAFE----E--NGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEG--------ATVTRGARVMEPHYVPMDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLL-K-----RG--VAYGAMFPGYTDTMHQANEFMSLDDLFRATAIYADAIYRLAK 4G1P Chain:A ((6-476)) --TSVFQKIDSLKPQFFSRLTKAIQIPAVSSDES------LRSKVFDKAKFISEQLSQSGFHDIKMVDLGIQPPPSTPNLSLPPVILSRFGSDPSKKTVLVYGHYDVQPAQLEDGWDTEPFKLVIDEAKGIMKGRGVTDDTGPLLSWINVVDAFKASGQEFPVNLVTCFEGMEESGSLKLDELIKKEANGYFKGVDAVCISDNYWLGTKKPVLTYG---------------------------------------------------------------------LRGCNYYQTIIEGPSADLHSGIFGGVVAEPMIDLMQVLGSLVDSKGKILIDGIDEMVAPLTEKEKALYKDIEFSVEELNAATGSKTSLYDKKEDILMHRWRYPSLSIHGVEGAFSAQGAKTVIPAKVFGKFSIRTVPDMDSEKLTSLVQKHCDAKFKSLNSPNKCRTE-LIHDGAYWVSDPFNAQFTAAKKATKLVYGVDPDFTREGGSIPITLTFQDALNTSVLLLPMGRGD-D---GAHSINEKLDISNFVGGMKTMAAYLQYYS-

General information: TITO was launched using: RESULT: Template: 4G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1781 24414 13.71 62.76 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 13.71 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1P-query.scw PDB file : Tito_Scwrl_4G1P.pdb: