Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFLKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIH-PDMYHIYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKI-PPSLRNIYKELYDDLGIAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIVFILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPIDWQLPETV 4WS3 Chain:A ((17-238)) ---LSELVERGWAAAL--EPVADQVAHMGQFLRAEIAAGRRYLPAGSNVLRAFTF-PFDNVRVLIVGQDPYPTPGHAVGLSFSVAPDVRPWPRSLANIFDEYTADLGYPLPSNGDLTPWAQRGVLLLNRVLTVRPSNPASHRGKGWEAVTECAIRALAARAAPLVAILWGRDASTLKPMLAAGNCVAIESPHPSPLSASRGFFGSRPFSRANELLVGMGAEPIDWRLP---

General information: TITO was launched using: RESULT: Template: 4WS3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1115 -17313 -15.53 -78.70 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -15.53 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_4WS3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WS3-query.scw PDB file : Tito_Scwrl_4WS3.pdb: