Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------MNDFTTEIVQTLVTKGDLNELF-----RSHLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEV-------NAFKS--RALNDKYVAIFMD----------ATYIPLKRQTVSK---EAIYIAIGIRE--DGTKEVLSYAIA-PTESTYVWNELLQDI--NSRGVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCC------IHVSRNIAHKVRVKDRKEICDDF---------------KAVYQANSKEE---ANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDF---------PPSIRRTIYSTNLIE--------SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSH--KRFSTGNRYISFNVY----------------------------------------------------------------------- 5SVD Chain:A ((5-589)) QMFFGVLDREELEYFKQAESTLQLDAFEAPEEKFQFVTSIIEEA--KGKELKLVTSQITSKLMERVILECDETQLKDIFQ-------------SFNGVFFGLSCHKYASHVLETLFVRSAALVERELLTY--------VTMENMFLF--MLNELKPHLKTMMNHQYASHVLRLLILILSSKTLPVYQTPESFKSELRDIITTLYKGFTNGAESRSDISQSTITKFREYSVDKVASPVIQLIIQVEGIFDRDRSFWRLVFNTADEKDPKEESFLEYLLSDPVGSHFLENVIGSARLKYVERLYRLYMKDRIVKLAKRDTTGAFVVRALLEHLKEKDVKQILDAVVPELSMLLNSNMDFGTAIINTSNKQGGYLRDDVIAQLIQKYYPEKSD-AKNILESCLLLSASTLGNTRDDWPTAEERRRSVFLEQLIDYDDKFLNITIDSMLALPEERLIQMCYHGVFSHVVEHVLQTTRVDIIKRKMLLNILSKESVNLACNVYGSHIMDKLWEFTAKLTLYKERIARALVLETEKVKNSIYGRQVWKNWKLELYVRKMWDWKKLIKEQEFEIFP

General information: TITO was launched using: RESULT: Template: 5SVD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1616 36133 22.36 98.99 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 22.36 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.315

(partial model without unconserved sides chains): PDB file : Tito_5SVD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5SVD-query.scw PDB file : Tito_Scwrl_5SVD.pdb: