Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIVPEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTE-VQFVKKLIEAGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGAGGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAG-DSKLKAMLDNGEEVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFLRSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA 2QM1 Chain:B ((5-322)) -DKKIIGIDLGGTTIKFAILTTDGVVQQKWSIETNILEDGKHIVPSIIESIRHRIDLYNMKKEDFVGIGMGTPGSVDIEKGTVVGAYNLNWTTVQPVKEQIESA-LGIPFALDNDANVAALGERWKGAGENNPDVIFITLGTGVGGGIVAAGKLLHGVAGCAGEVGHVTVDPNGFDCTCGKRGCLETVSSATGVVRVARHLSEEFAGDSE-LKQAIDDGQDVSSKDVFEFAEKGDHFALMVVDRVCFYLGLATGNLGNTLNPDSVVIGGGVSAAGEFLRSRVEKYFQEFTFPQVRNSTKIKLAELGNEAGVIGAASLALQF-

General information: TITO was launched using: RESULT: Template: 2QM1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1897 -40669 -21.44 -128.70 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.95 3D Compatibility (PKB) : -21.44 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_2QM1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QM1-query.scw PDB file : Tito_Scwrl_2QM1.pdb: