Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNYLDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV-QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSVITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQDLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLTLFGYWCYRKFKRQKQRKKNNR 3G69 Chain:B ((3-199)) -----------------------------------------------EVIKEFDETVSQMDKAELEERWRLAQAFNATLK----PS---EILDPFTEQEKKKGSEYANMLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHRGLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATLLTCTPYMINSHRLLVRGKRIPYTAPI------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1002 -25048 -25.00 -128.45 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -25.00 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_3G69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G69-query.scw PDB file : Tito_Scwrl_3G69.pdb: