Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDMIWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVSTEHEWFKKALAG-DQHYQDFFILRE-----EPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQADLNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKD--------EQLKNNKENDGKPEYTDKPITHTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYENGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFV-AIDQYRKQGAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQG-FTEKEAFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSYYKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPEGFTEANILID---NYSDTVLHQSLELKPYQAIALLKN 4GI8 Chain:B ((7-551)) --WKSAVFYQVYPRSFKDTNGDGIGDFKGLTEKLDYLKGLGIDAIWINPHYASPNTDNGYDISDYREVMKEYGTMEDFDRLMAELKKRGMRLMVDVVINHSSDQHEWFKSSRASKDNPYRDYYFWRDGKDGHEPNNYPSFFGGSAWEKDPVTGQYYLHYLGRQQPDLNWDTPKLREELYAMLRFWLDKGVSGMRFDTVATYSKTPGFPDLTPEQMKNFAE-----AYTQGPNLHRYLQEMHEKVF-DHYDAVTAGEIFGAPLNQVPLFIDSRRKELDMAFTFDLIRYD-RALDRWHTIPRTLADFRQTIDK-VDAIAGEYGWNTFFLGNHDNPRAVSHFGDDRPQWREASAKALATVTLTQRGTPFIFQGDELGMTNYPFKTLQDFDDIEVKGFFQDYVETGKATAEELLTNVALTSRDNARTPFQWDDSANAGFTTGKPWLKVNPNYTEINAAREIGDPKSVYSFYRNLISIRHETPALSTGSYRDIDPSNADVYAYTRSQDGETYLVVVNFKAEPRSFTLPDGMHIAETLIESSSPAAPAAGAASLELQPWQS------

General information: TITO was launched using: RESULT: Template: 4GI8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3002 82077 27.34 156.04 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : 27.34 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4GI8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GI8-query.scw PDB file : Tito_Scwrl_4GI8.pdb: