Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQGYLRKNG-KVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDL-GQFDHITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVP---------------LSKLNVGLKCGGSDGLSGIT----------ANPLLGAFSDYLIAQGGSTIL-------------TEVPEMFGAEQVL---MARAENEEVFDSIVDLINDFKQYFLSY----GEPVYENPSPGNKAGG-------ITTLEDKSLGCTQ--KSGSSPVVDVLQYGEKIR-KPGLSLL-----QAPGNDLVAASALASSDCQLVLFTTGR--GTP-----FGSYVPTVKVSTNTTLFERKGHWMDFNAG-------VLLEQPMEVILESFVQKIIAVASG---------EETKNEQNDVREIAIFKNGVTL---------------------------------------- 5AWO Chain:A ((15-610)) -PVTAAPPLRLASRNSVFTRSGAGP--RYWNIYGYSFPHNAPIPENEWKVNIDWLAG-NFADFGYDIACTDGWIEGSS-------RTTGNGYIT------SYNDSWQHDWAYWANYLAARKMKLGVYYNPLWVH-RAAVEDASKTVLGRPDVKIADLVVPGDFFARDIGGNQLYWLDVTKSGAKEYVQGYVRYFKDLGVPYLRID-----------FLSWYEDGRDANIGQVNAPHGRANYELALSWINEAAGEDMEVSLVPHMFQDGSAELANGDLVRINADADKGGWDRLSGMRQNWQDAWPNWANPFCGFTGWSHRNGRGQLILDGDFMRASTFASDEERKTMMNLMVAAGSPLAIADTYQQIGNNAWVYTNKEVLQLNADGLVGKPLYRSATPFSKDPGSRDTERWAGQLPDGSWGVALFNRSDTETVTKTIDFAKDLGLATGGNVRDLWEHRNLGMDSRATAALAPHASAIFRVTPPKMHGTTRYPAAFAAWGGGAGFNYNHPGYDGNGFVDGLQAGSGSADPLVTFAVQVPHRGSYAIRYRYANATGDTSTMTVTAEKADRSTVDGPVHVSFPGLATWDTWGVADGTITLDAGLNLVTIGRGATDKGAINLNWIELDM

General information: TITO was launched using: RESULT: Template: 5AWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2824 67791 24.01 144.24 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 24.01 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_5AWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AWO-query.scw PDB file : Tito_Scwrl_5AWO.pdb: