Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNTIFLQRVADLYDTFKQHDSQQSDRLQRYRNIEVESAQLISQLIRMQQAKSILEIGTSTGYSTLWLAEAAQATGGQVITVEIDAKRSAEAKRHVAELELSEIVQFWVGDAADYLKA-AQEKFDFILLDAERDAYENYWPDLKRLMKPKGGVLVVDNVISHA-----------------AEVNRFIALIKKDPDYMSSILPVGAGLCLVVTK 3NTV Chain:B ((51-231)) -----------------------------EVPIVDRLTLDLIKQLIRMNNVKNILEIGTAIGYSSMQFASISD--DIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQ-GLVITDNVLYHGFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKG

General information: TITO was launched using: RESULT: Template: 3NTV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 731 -52239 -71.46 -320.48 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -71.46 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_3NTV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NTV-query.scw PDB file : Tito_Scwrl_3NTV.pdb: