Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKTLQFGITNFEIDFKDLVMNTMIKNKRFENDVEFRGADIKDSRAVVILLHGRRQNADTIYELAEKI--GFTDITYILPLAPELT-----------WYPRGFMR-DLEDNQPYLSVALERIHYIITDLKNQNIKPENIFLMGFSQGACIVSQYLWEN-PQKLGGVIAFTGGLFGPELTYTSHEGLPINGTQLIFTGGKEDSWVPEQRVRDTAALFEKLGGNVTTKIYSGRDHLVSDEEVELAKTLLNI-- 1AUO Chain:A ((1-218)) MTEPLILQPA-------------------------------KPADACVIWLHGLGADRYDFMPVAEALQESLLTTRFVLPQAPTRPVTINGGYEMPSWYDIKAMSPARSISLEELEVSAKMVTDLIEAQKRTGIDASRIFLAGFSQGGAVVFHTAFINWQGPLGGVIALSTYA--PTFGDELELSASQQRIPALCLHGQYDDVVQNAMGRSAFEHLKSRGVTVTWQEYPMGHEVLPQEIHDIGAWLAARLG

General information: TITO was launched using: RESULT: Template: 1AUO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1094 -29636 -27.09 -147.44 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -27.09 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_1AUO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AUO-query.scw PDB file : Tito_Scwrl_1AUO.pdb: