Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRQVLILASSQSLFQTVSVMVMTIGGLAGANIANTPTLSTLPIASMFLGTALMMFPASILMAHIGRRNGFLFGAFLGVLGGIIASIG---------------IFYSSLSLLALGTLCVGAYQSFAQFY-RFAASEVANDAFRSRAISWVLAGGIVAALIGPTLARFGGPLF-----QHLEYIGSFLIISIISLVAMGILSSLHIADT-VE--QK-SD-FT-------------AGRPW----QQIVFQPTYLV-A---------LFGAITGYGIMILGMTATPIAMRHSHHELGSITTVIQLHVLGMFLPSFFTGNLIARFGVLKIMFAGLLLFACYIGFALSG-----IQFTSFAISLILLGVGWNFLFIGSTSLLTGTYSVAEKAKAQAINDMTVFVVGLICSFSA------GALLDLFGWKAMNIALIPWLVIAAASLFWLSQKREKQFV 4GC0 Chain:A ((52-458)) ----------------------------------AANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALF-------------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1303 -181961 -139.65 -530.50 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -139.65 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: