Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEARVIGL-EKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTADAYREFLDQ-----SGLNARIQAELAELNVDD---------------------------------------VNALAETGAKIRQWIVET--PLTPGLEQEIREAFAALSNGNPDIAVAVRSSATAEDLPDASFAGQQETFLNIR------------GIDNVLIAVKEVFASLFNDRAIAYRVHQGFEHSLVALSAGVQRMVRSETG---AAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSKPLLNAGKHAVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQERQQFALNDHELQELAKQALIIEKHYG---APMDIEWAKDGDDGQIYIVQARPETVKSRQNVGTMERYL---LKQKGTVLCEGRSIGQRIGSGKVRI-VNSIKEMDKVQDGDVLVSDMTDPDWEPVMKRAAAIITNRGGRTCHAAIITRELGVPAIVGCGNATE------------VLTDGQEVTVSCAEGDTGFIYEGAL------DFEVQRNSIESMPKLSFKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIESLPRETRAAVMTRTAGYASPVEFYVEKLVEGIATLAAAFADKPVIVRMSDFKSNEY------------------ANLIGGKLYEPEEENPMLGFRGASRYI--SDNFRDCFELECRALKKVRDEMGL-TNIQIMIPFVRTVSEAKRVIELLAQNGLKRGEN-----GLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLFDERD-------------AAVKALLSMAIHACRKAGK--YVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAEEKIKQI 1KBL Chain:A ((1-872)) --AKWVYKFEEGNASMRNLLGGKGCNLAEMTI----LGMPIPQGFTVTTEACTEYYNSGKQITQEIQDQIFEAITWLEELNGKKFGDTEDPLLVSVRSGARASMPGMMDTILNLGLNDVAVEGFAKKTG-NPRFAYDSYRRFIQMYSDVVMEVPKSHFEKIIDAMKEEKGVHFDTDLTADDLKELAEKFKAVYKEAMNGEEFPQEPKDQLMGAVKAVFRSWDNPRAIVYRRMNDIPGDW-GTAVNVQTMVFGNKGETSGTGVAFTRNPSTGEKGIYGEYLINAQGEDVVAGVRTPQPITQ-------------------------LENDMPDCYKQFMDLAMKLEKHFRDMQDMEFTIEEGKLYFLQTRNGKRTAPAALQIACDLVDEGMITEEEAVVRIEAKSLDQLLHPTFNPAALKAGEVIGSALPASPGAAAGKVYFTADEAKAAHEKGERVILVRLETSPEDIEGMHAAEGILTVRGGMTSHAAVVARGMGTCCVSGCGEIKINEEAKTFELGGHTFAEGDYISLD-GSTGKIYKGDIETQEASVS-GSFERIMVWADKFRTLKVRTNADTPEDTLNAVKLGAEGIGLCRTEHMFF-----------EADRIMKIRKMILSDSVEAREEALNELIPFQKGDFKAMYKALEGRPMTVRYLDPPLHEFVPHTEEEQAELAKNMGLTLAEVKAKVDELHEFNPMMGHRGCRLAVTYPEIAKMQTRAVMEAAIEVKEETGIDIVPEIMIPLVGEKKELKFVKDVVVEVAEQVKKEKGSDMQYHIGTMIEIPRAALTADAIAEEAEFFSFGTNDLTQMTFGFSRDDAGKFLDSYYKAKIYESDPFARLDQTGVGQLVEMAVKKGRQTRPGLKCGICGEHGGD-PSSVEFCHKVGLNYVSCSPFRVPIARLAAAQAALNN-

General information: TITO was launched using: RESULT: Template: 1KBL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4225 140982 33.37 189.49 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 33.37 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.264

(partial model without unconserved sides chains): PDB file : Tito_1KBL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KBL-query.scw PDB file : Tito_Scwrl_1KBL.pdb: