Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRHYTGIDQLINSFDQALRSLVPGATAAQRQNPAETVEAKLGVEDARHVAGLMRVNHSGEVCAQALYHGQALTAKL---PNVRREMQQAAIEEQDHLAWCEDRLKELNSHTSLLN-PIWYGLSYGMGALAGIAGDKYSLGFVAETERQVSLHLQDHLNQLPAQDERSRKILEQMNEDELHHRHTALEAGGVELPYAVKITMTAISKLMTKTSYYL 2YJK Chain:C ((11-156)) --------------------------------------LTADPEVAAAAAQFLTPVVHKMQALVVNGKQAHWNVRGSNFIAIHELLDSVVAHAQDYADTAAERIVALGLPIDSRVSTMAEKTSTAV--PAGFAQWQDEIKAIVSDIDAALVDLQAAIDGLDEVDLTSQDVAIEIKRGVDKDRWFLL-----------------------------

General information: TITO was launched using: RESULT: Template: 2YJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 609 -24133 -39.63 -169.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.57 3D Compatibility (PKB) : -39.63 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_2YJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YJK-query.scw PDB file : Tito_Scwrl_2YJK.pdb: